Fluorescein NHS ester

6-Fluorescein N-hydroxysuccinimide ester

Product Specifications

Formula:
C25H15NO9
M.W.:
473.39
F.W.:
358.31
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Fluorescein is a bright fluorophore that is utilized in a wide range of applications and is especially popular for oligonucleotide labeling. Most syntheses directly incorporate fluorescein using the phosphoramidite approach, and this NHS ester provides the option for post-deprotection labeling. When paired with a 5’-amino-modifier C6 (10-1906, 10-1907, 10-1916 or 10-1947), the resulting product would be identical to that generated with 5’-Fluorescein Phosphoramidite (10-5901).

Details

Usage

  • Coupling: For labeling 0.2 µmoles of an amino-modified oligonucleotide, dissolve 2.4 mg of the Fluorescein NHS Ester in 100 µL DMSO (or DMF). Dissolve the amino-modified oligonucleotide in 200 µL 0.25 M carbonate/bicarbonate buffer (pH 9). Add 20 µL of the NHS Ester solution and vortex. Let react for 60 min at room temperature and desalt.
Specifications
Diluent DMF or DMSO
Storage Freezer storage, -10 to -30°C, dry