Coumarin Azide

Glen Research is offering first our most popular labels for general interest and, subsequently, we will add azide products that are not compatible with phosphoramidite chemistry. 7-Hydroxycoumarin, also known as umbelliferone, is a highly fluorescent, pH-sensitive fluorophore that emits in the blue region of the spectrum. However, its fluorescence is strongly quenched if the hydroxyl is alkylated or phosphorylated, making it useful in high-throughput screening for phosphatases and lipases. Interestingly, it was found that the 3-azido derivative is also highly quenched but, upon reaction with an alkyne in the presence of copper to form the triazole, the fluorescence is restored.1 The clicked coumarin emits at a lambda max of 480 nm and absorbs at 358 nm. The azides are offered in 25 and 100 µmole packs for convenient oligonucleotide labelling.