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MGB Eclipse® CPG - (20-5927)

5-(6-(6-(6-(4-N-(3-Dimethoxytrityloxypropyl)-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanoyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carboxamido)pentyl-1-O-diglycoloyl long chain alkylamino CPG

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20-5927

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Product Specifications

F.W.:

1120.56

Pore Size:

1000Å

Description

MGB Eclipse® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization while the Eclipse® has a broad absorption range that quenches many of the most common fluorophores including FAM, HEX, TET and Yakima Yellow®. Together, MGB Eclipse® is an attractive group for the synthesis of hydrolysis probes, the most common probe detection method for qPCR.

Details

Usage

Coupling: Regular with UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx and Cap A 40-4210-xx/40-4212-xx). Use 0.5 M CSO in ACN for oxidation (Catalog Number 40-4632-xx) using a 3 minute oxidation time.
Deprotection: Cleave and deprotect the oligonucleotide in 30% Ammonium Hydroxide for 2 hours at Room Temperature. If standard monomers and iodine oxidation are used, acceptable results can be obtained when deprotected in NH₄OH/EtOH 3:1 (v/v) for 17 hr at 55°C.