MGB Eclipse® CPG

5-(6-(6-(6-(4-N-(3-Dimethoxytrityloxypropyl)-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanoyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carboxamido)pentyl-1-O-diglycoloyl long chain alkylamino CPG

Product Specifications

F.W.:
1120.56
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Description

MGB Eclipse® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization while the Eclipse® has a broad absorption range that quenches many of the most common fluorophores including FAM, HEX, TET and Yakima Yellow®. Together, MGB Eclipse® is an attractive group for the synthesis of hydrolysis probes, the most common probe detection method for qPCR.

Details

Usage

  • Coupling: Regular with UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx and Cap A 40-4210-xx/40-4212-xx). Use 0.5 M CSO in ACN for oxidation (Catalog Number 40-4632-xx) using a 3 minute oxidation time.
  • Deprotection: Cleave and deprotect the oligonucleotide in 30% Ammonium Hydroxide for 2 hours at Room Temperature. If standard monomers and iodine oxidation are used, acceptable results can be obtained when deprotected in NH4OH/EtOH 3:1 (v/v) for 17 hr at 55°C.
Specifications
Storage Freezer storage, -10 to -30°C, dry

Intellectual Property

This product is sold under licensing arrangements between ELITechGroup Inc. and Glen Research. The purchase price of this product includes limited, nontransferable rights to use the product solely for activities of the purchaser which are directly related to human diagnostics. Other uses, including incorporation of the product into another commercial product, are prohibited without additional license rights. For information on purchasing a license to this product for purposes other than those stated above, contact:

ELITech Group Molecular Diagnostics, 21720 23rd Drive SE, Suite 150,
Bothell, WA 98021.
Phone (425) 482-5555.
Fax (425) 482-5550.
Email: [email protected]

This limited license permits the person or legal entity to which this product has been provided to use the product, and the data generated by use of the product, only for human diagnostics. Neither ELITechGroup Inc. nor its licensors grants any other licenses, expressed or implied for any other purposes.

Some components of nucleic acid analysis, such as specific methods and compositions for manipulating or visualizing nucleic acids for analysis, may be covered by one or more patents owned by other parties. Similarly, nucleic acids containing specific nucleotide sequences may be patented. Making, using, offering for sale, or selling such components or nucleic acids may require one or more licenses. Nothing in this document should be construed as an authorization or implicit license to make, use or sell any so covered component or nucleic acid under any such patents.