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DesthiobiotinTEG-CPG - (20-2952)

1-Dimethoxytrityloxy-3-O-(N-desthiolbiotinyl-3-aminopropyl)-triethyleneglycolyl-glyceryl-2-O-succinyl-long chain alkylamino-CPG

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20-2952

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Product Specifications

F.W.:

539.56

Pore Size:

1000Å

1-Dimethoxytrityloxy-3-O-(N-desthiolbiotinyl-3-aminopropyl)-triethyleneglycolyl-glyceryl-2-O-succinyl-long chain alkylamino-CPG

Description

Biotin-dT can replace dT residues within the oligonucleotide sequence. 5'-Biotin phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5'-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3' terminus. Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.

Details

Usage

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.