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2'-F-I-CE Phosphoramidite - (10-3440)

5'-Dimethoxytrityl-deoxyInosine, 2'-fluoro-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3440

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Product Specifications

Formula:

C₄₀H₄₆FN₆O₇P

M.W.:

772.82

F.W.:

332.18

CAS Number:

2245842-16-2

5'-Dimethoxytrityl-deoxyInosine, 2'-fluoro-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

The significance of inosine in oligonucleotide synthesis comes from its ability to base pair with each of the standard bases (A, C, G, and T/U). Due to these unique pairing properties, inosine is considered as a “Universal Base” and has been an attractive candidate for many applications involving either synthetic primers or probes. This 2’-F version of inosine can be used in the same way as standard 2’-F phosphoramidites.

Details

Usage

Coupling: 3 minutes coupling time recommended.
Deprotection: Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature. (Note - heating in AMA will lead to some degradation of the 2'-Fluoro nucleotides.)