Quasar® 570-dT Phosphoramidite

5'-Dimethoxytrityloxy-5-[(N-(6-(N1-ethyl-3,3,3',3'-tetramethylindocarbocyanin-1'-yl) hexanoyl)-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite, hexafluorophosphate salt

Product Specifications

Formula:
C79H100F6N8O10P2
M.W.:
1497.65
F.W.:
911.05
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Quasar®570 and Quasar®670 dyes are two cyanine derivatives of Cyanine 3 and Cyanine 5, respectively. These two fluorophores differ in structure simply by the number of carbons in the conjugated polyene linkage. Cyanine dyes are a popular oligonucleotide modification and are commonly used for imaging, flow cytometry, and genomic applications. With Quasar®570-dT and Quasar®670-dT, these dyes can be incorporated internally without disrupting base pairing or stacking. Monomers can be inserted as a replacement for a dT residue.

 

Particularly for Quasar® 670-dT, the use of UltraMILD monomers is preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMILD Cap Mix A (40-4210-xx/40-4212-xx). 0.02M Iodine for oxidation is recommended.

Details

Usage

  • Coupling: 10 minute coupling time recommended. Use 0.02M Iodine to avoid degradation of the cyanine dye.
  • Deprotection: If dmf-dG is used during synthesis, deprotect in Ammonium Hydroxide for 2 hours at 65 °C; with ibu-dG, deprotect for 24-36 hours at room temperature. Note Quasar 570 is compatible with UltraFast deprotection (AMA 10 minutes at 65 °C) if Ac-dC was used.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-5953-95 50 µmol 0.08 0.5 3.33 2 1.25 0.91 0.67 0.17
10-5953-90 100 µmol 0.15 1 20 12 7.5 5.45 4 1
10-5953-02 0.5 g 0.25 1.67 42.33 25.4 15.88 11.55 8.47 2.12

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-5953-95E 50 µmol 0.08 0.75 0.07 8.6 5.38 3.91 0.54
10-5953-90E 100 µmol 0.15 1.5 0.07 23.6 14.75 10.73 1.48
10-5953-02E 0.25 g 0.25 2.49 0.07 43.4 27.13 19.73 2.71