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DesthiobiotinTEG Phosphoramidite - (10-1952)

1-Dimethoxytrityloxy-3-O-(N-desthiobiotinyl-3-aminopropyl)-triethyleneglycolyl-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

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10-1952

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Product Specifications

Formula:

C₅₂H₇₈N₅O₁₁P

M.W.:

980.19

F.W.:

539.56

1-Dimethoxytrityloxy-3-O-(N-desthiobiotinyl-3-aminopropyl)-triethyleneglycolyl-glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Description

Biotin-dT can replace dT residues within the oligonucleotide sequence. 5'-Biotin phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5'-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3' terminus. Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.

Details

Usage

Coupling: 12-15 minute coupling time. To maintain label yield, carry out the synthesis DMT-On.
Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with ACN. At this point, the DMT group may be safely removed without loss of label during deprotection.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1952-02	0.25 g	.25grams	2.55	71.67	43	26.88	19.55	14.33	3.58
10-1952-90	100 µmol	.098grams	1	20	12	7.5	5.45	4	1
10-1952-95	50 µmol	.049grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1952-02	0.25 g	.25grams	3.81	0.07	69.8	43.63	31.73	4.36
10-1952-90	100 µmol	.098grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1952-95	50 µmol	.049grams	0.75	0.07	8.6	5.38	3.91	0.54