Biotin-dT can replace dT residues within the oligonucleotide sequence. 5'-Biotin phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5'-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3' terminus.
Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.
Coupling: 12-15 minute coupling time. To maintain label yield, carry out the synthesis DMT-On.
Deprotection: To maintain label yield as a 5' modifier, cleave and deprotect as required by nucleobases and then remove the DMT group. Alternatively, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups and rinse with ACN. At this point, the DMT group may be safely removed without loss of label during deprotection.
Freezer storage, -10 to -30°C, dry
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.