Methacrylate C6 Phosphoramidite is a terminus modifier that attaches a methacrylate functional group to an oligonucleotide. Methacrylate-modified oligonucleotides can be reacted with thiol-modified surfaces or incorporated into polyacrylamide gels. Methacrylate-modified oligonucleotides have been used to generate oligonucleotide labeled hydrogels, microspheres, microarrays, and functionalized polymeric surfaces, among other applications. These oligonucleotide-labeled surfaces and polymers have been used for purification, hybridization detection, PCR amplification, affinity capture, aptamers, sequencing, and biosensors. Recent applications of methacrylate-labeled oligonucleotides include aptamer-based hydrogels, molecular imprinting, and Expansion Microscopy.
| Specifications | |
|---|---|
| Diluent | Acetonitrile, anhydrous |
| Storage | Freezer storage, -10 to -30°C, dry |
| Stability | 2-3 days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1891-90 | 100 µmol | 0.04 | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1891-02 | 0.25 g | 0.25 | 6.49 | 203 | 121.8 | 76.13 | 55.36 | 40.6 | 10.15 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1891-90E | 100 µmol | 0.04 | 1.5 | 0.07 | 23.6 | 14.75 | 10 | 1.48 |
| 10-1891-02E | 0.25 g | 0.25 | 9.68 | 0.07 | 187.2 | 117 | 85.09 | 11.7 |
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