dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C46H52N5O8P
M.W.:
833.93
F.W.:
289.18
CAS Number:
102212-98-6
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination, and each vial type is thoroughly tested or inspected to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Normal

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1010-02 0.25 g .25grams 3 86.67 52 32.5 23.64 17.33 4.33
10-1010-05 0.5 g .5grams 6 186.67 112 70 50.91 37.33 9.33
10-1010-10 1.0 g 1grams 11.99 386.33 231.8 144.88 105.36 77.27 19.32
10-1010-20 2.0 g 2grams 23.98 786 471.6 294.75 214.36 157.2 39.3
10-1010-40 4.0 g 4grams 47.97 1585.67 951.4 594.63 432.45 317.13 79.28

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1010-C2 0.25 g .25grams 4.47 0.07 86.2 53.88 39.18 5.39
10-1010-C5 0.5 g .5grams 8.95 0.07 175.8 109.88 79.91 10.99
10-1010-1C 1.0 g 1grams 17.9 0.07 354.8 221.75 161.27 22.18
10-1010-2C 2.0 g 2grams 35.8 0.07 712.8 445.5 324 44.55