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1-Methyl-PseudoUridine Phosphoramidite - (10-3056)

5'-Dimethoxytrityl-1-methyl-pseudoUridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3056

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	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₄₆H₆₃N₄O₉PSi

M.W.:

875.07

F.W.:

320.19

CAS Number:

875302-45-7

5'-Dimethoxytrityl-1-methyl-pseudoUridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

RNA methylation occurs in a large selection of RNA nucleosides and this post transcriptional modification of RNA, carried out by a variety of RNA methyltransferases, appears in a wide variety of RNA species - including tRNA, mRNA, miRNA and RNA viruses. Over 90 methylated nucleosides have been found in tRNA and these play many significant roles in tRNA structure. In addition, methylation appears to mark the tRNA as mature, preventing its degradation as well as directing localization within the cell. mRNA, modified with 1-methylpseudouridine (1-Me-Ψ ) alone or in combination with 5-methylcytidine (5-Me-C), significantly increases protein expression in cells and mouse models. 1-Me-Ψ is also a modified nucleobase that can greatly enhance the properties of mRNA by reducing immunogenicity and increasing stability.

Details

Usage

Coupling: 12 minute coupling time recommended.
Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3056-02	0.25 g	.25grams	2.86	82	49.2	30.75	22.36	16.4	4.1
10-3056-90	100 µmol	.088grams	1	20	12	7.5	5.45	4	1
10-3056-95	50 µmol	.044grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3056-02	0.25 g	.25grams	4.26	0.07	78.8	49.2	16.42	4.9
10-3056-90	100 µmol	.088grams	1.5	0.07	23.6	14.7	4.92	1.4
10-3056-95	50 µmol	.044grams	0.75	0.07	8.6	5.3	1.79	0.5