Home Applications Probing DNA Structure with Fluorescent Nucleosides Ribo-tC° Phosphoramidite

Ribo-tC° Phosphoramidite - (10-3517)

5'-O-(4,4'-Dimethoxytrityl)-1'-(1,3-diaza-2-oxophenoxazin-1-yl)-2’-O-TBDMS-β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3517

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Product Specifications

Formula:

C₅₁H₆₄N₅O₉PSi

M.W.:

950.16

F.W.:

395.26

CAS Number:

1188522-62-4

5'-O-(4,4'-Dimethoxytrityl)-1'-(1,3-diaza-2-oxophenoxazin-1-yl)-2’-O-TBDMS-β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

The bright fluorescent tricyclic cytosine analogues tC and tCO stand out among fluorescent bases due to their virtually unquenched fluorescence inside single- or double-stranded DNA. Until recently, this family of tricyclic cytosines had only been studied and used in DNA contexts and, importantly, introduced as possible donors of the first DNA base analogue FRET-pair with tCnitro. Fluorescent base analogues for RNA are limited in number compared to their DNA counterparts. To facilitate the application of such analogues, characterization of their structural and dynamics behavior in RNA compared to the corresponding natural nucleoside is important. We now introduce the tCO ribonucleoside, which has been incorprated into a range of RNA sequences, where it was shown to be a very potent and useful fluorophore in this context.1 Glen Research offers this useful fluorescent ribonucleoside analogue in cooperation with ModyBase HB.

Details

Usage

Coupling: 12 minute coupling time recommended.
Deprotection: As required by nucleobases. Compatible with ammonium hydroxide/ethanol 3:1 (v/v) for 17 hours at room temperature, AMA (ammonium hydroxide/40% methylamine 1:1 (v/v)) for 10 minutes at 65°C or UltraMild deprotection conditions.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3517-02	0.25 g	0.25	2.63	74.33	44.6	27.88	20.27	14.87	3.72
10-3517-90	100 µmol	0.1	1	20	12	7.5	5.45	4	1
10-3517-95	50 µmol	0.05	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3517-02	0.25 g	0.25	3.93	0.07	72.2	45.13	32.82	4.51
10-3517-90	100 µmol	0.1	1.5	0.07	23.6	14.75	10.73	1.48
10-3517-95	50 µmol	0.05	0.75	0.07	8.6	5.38	3.91	0.54