BBQ-650® CPG - (20-5934)
5-O-(Dimethoxytrityl)-1-[9-[4-nitro-2',5'-dimethoxy-azobenz-4'-yl)-diazo]-julolidine-8]-O-pentyl-3-O-glycolate-CPG
5-O-(Dimethoxytrityl)-1-[9-[4-nitro-2',5'-dimethoxy-azobenz-4'-yl)-diazo]-julolidine-8]-O-pentyl-3-O-glycolate-CPG
We are happy to offer several products containing the BlackBerry. Quencher (BBQ-650.), which exhibits a broad absorption profile from 550nm to 750nm, centered at 650nm. This range offers more effective quenching of some of our popular long wavelength dyes like TAMRA, Redmond Red, Cy dyes and DyLight dyes. We offer BBQ-650 products for the 3' and 5' termini, as well as BBQ-650-dT for inclusion within the oligonucleotide sequence, with the following properties:
- Quenches the fluorescence of long wavelength dyes
- Quenches in FRET and contact mode
- Absorbance maximum at ~650nm
- Quenching range - 550-750nm
- Compatible with standard oligo synthesis chemistry
- Compatible with regular deprotection but requires mild deprotection with AMA at room temperature
- Available for 3', 5', and internal substitution
- More stable than BHQ-3
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: Complete the deprotection using the protocol required by the nucleobases. Compatible with standard deprotection conditions in Ammonium Hydroxide and room temperature AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) for 2 hours.
| Specifications | |
|---|---|
| Recommended Storage | Freezer storage, -10 to -30°C, dry. Light sensitive material. |
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