Home Products DNA & RNA Nucleosides, Analogs, and Supports Nucleoside Analog Phosphoramidites DNA Nucleoside Analogs Analog Phosphoramidites for Duplex Stabilization Bases Affecting Duplex Stability N4-Ac-N4-Et-dC-CE Phosphoramidite

N4-Ac-N4-Et-dC-CE Phosphoramidite - (10-1513)

5'-Dimethoxytrityl-N4-acetyl-N4-ethyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

As low as $135.00

In stock

Only %1 left

default

10-1513

Product	Options
	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₄₃H₅₄N₅O₈P

M.W.:

799.89

F.W.:

317.24

CAS Number:

1377950-52-1

5'-Dimethoxytrityl-N4-acetyl-N4-ethyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Sequences with high GC content may contain mismatches and still hybridize because of the high stability of the G-C base pair. The N4-ethyl analogue of dC (N4-Et-dC) hybridizes specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair. Coupling N6-Me-dA (10-1003) and N4-Et-dC (10-1068) with 1H-tetrazole leads to a trace of branching at the secondary amine positions, while DCI leads to around 15% branching. In collaboration with Berry and Associates, the acetyl protected monomers were prepared. Acetyl protection was chosen since it would block branching reactions. Oligonucleotides synthesized using these monomers proved to be compatible with all popular deprotection strategies from UltraMild to UltraFast. When the acetyl protected monomers were compared with the unprotected monomers using DCI as activator, branching was reduced from 15% to zero.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1513-02	0.25 g	.25grams	3.13	91	54.6	34.13	24.82	18.2	4.55
10-1513-90	100 µmol	.08grams	1	20	12	7.5	5.45	4	1
10-1513-95	50 µmol	.04grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1513-02	0.25 g	.25grams	4.66	0.07	86.8	54.25	39.45	5.43
10-1513-90	100 µmol	.08grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1513-95	50 µmol	.04grams	0.75	0.07	8.6	5.38	3.91	0.54