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N3-Methyl-C-CE Phosphoramidite - (10-3013)

5'-Dimethoxytrityl-N4-acetyl-N3-methyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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10-3013
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Product Specifications

Formula:
C48H66N5O9PSi
M.W.:
916.14
F.W.:
319.21
CAS Number:
2931457-60-0
5'-Dimethoxytrityl-N4-acetyl-N3-methyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Details

Usage

  • Coupling: Coupling: 15 minute coupling time recommended
  • Deprotection: Deprotection: RNA Deprotection-see bulletin
    Note: This product is not compatible with methylamine deprotection reagents such as AMA, EMAM, and methylamine gas.
Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v)
Recommended Storage Freezer storage, -10 to -30°C, dry
Stability In Solution 1-2 Days
Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3013-95 50 µmol 0.46 0.5 3.3 2 1.2 0.9 0.6 0.1
10-3013-90 100 µmol 0.92 1 20 12 7.5 5.4 4 1
10-3013-02 0.25 g 0.25 2.73 77.6 46.6 29.1 21.1 15.5 3.8

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3013-95 50 µmol 0.46 0.75 0.07 8.6 5.3 1.7 0.5
10-3013-90 100 µmol 0.92 1.5 0.07 23.6 14.7 4.9 1.4
10-3013-02 0.5 g 0.25 4.07 0.07 75 46.8 15.6 4.6
Certificate of analysis

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