N3-Methyl-C-CE Phosphoramidite - (10-3013)
5'-Dimethoxytrityl-N4-acetyl-N3-methyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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10-3013
5'-Dimethoxytrityl-N4-acetyl-N3-methyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Details
Usage
- Coupling: Coupling: 15 minute coupling time recommended
- Deprotection: Deprotection: RNA Deprotection-see bulletin
Note: This product is not compatible with methylamine deprotection reagents such as AMA, EMAM, and methylamine gas.
Specifications | |
---|---|
Diluent | Anhydrous Acetonitrile/Dichloromethane 1:1 (v/v) |
Recommended Storage | Freezer storage, -10 to -30°C, dry |
Stability In Solution | 1-2 Days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
---|---|---|---|---|---|---|---|---|---|
LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
10-3013-95 | 50 µmol | 0.46 | 0.5 | 3.3 | 2 | 1.2 | 0.9 | 0.6 | 0.1 |
10-3013-90 | 100 µmol | 0.92 | 1 | 20 | 12 | 7.5 | 5.4 | 4 | 1 |
10-3013-02 | 0.25 g | 0.25 | 2.73 | 77.6 | 46.6 | 29.1 | 21.1 | 15.5 | 3.8 |
Expedite
Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
---|---|---|---|---|---|---|---|---|
Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
10-3013-95 | 50 µmol | 0.46 | 0.75 | 0.07 | 8.6 | 5.3 | 1.7 | 0.5 |
10-3013-90 | 100 µmol | 0.92 | 1.5 | 0.07 | 23.6 | 14.7 | 4.9 | 1.4 |
10-3013-02 | 0.5 g | 0.25 | 4.07 | 0.07 | 75 | 46.8 | 15.6 | 4.6 |
Technical documents
Certificate of analysis
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