Oligonucleotide Deprotection Guide

Deprotection of oligonucleotides can be visualized in three parts: cleavage, phosphate deprotection, and base deprotection. This process is quite straightforward for simple oligos, but complex or modified oligos require careful evaluation of deprotecting conditions.

The primary consideration to heed when establishing deprotection procedures for any oligo is First, Do No Harm. This begins with a review of the components of the oligonucleotide to acknowledge any sensitivity or pre-treatment requirements. You can then proceed with confidence to deprotect to completion!

Our guide provides readers with a practical overview to understanding the various considerations before choosing the optimal deprotection strategy.

Topics covered:

• Deprotect to Completion
• RNA Deprotection
• Dye-Containing Oligonucleotides
• Alternatives to Ammonium Hydroxide
• On-Column Deprotection of Oligonucleotides in Organic Solvents