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2'-OMe-N6-Me-A-CE Phosphoramidite - (10-3105)

5'-Dimethoxytrityl-N-methyl-N-phenoxyacetyl-Adenosine,2'-O-methyl- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3105

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Product Specifications

Formula:

C₅₀H₅₈N₇O₉P

M.W.:

932.03

F.W.:

357.28

5'-Dimethoxytrityl-N-methyl-N-phenoxyacetyl-Adenosine,2'-O-methyl- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

2’-OMe-N6-Me-A, commonly referred to as ^m6A_m, is a crucial component of the 5’-cap of mRNA. 2’-OMe-A is often the first transcribed nucleotide, and this modification is a result of methylation at the N6-position of the nucleobase. The 5’-cap structures initiate translation and protect RNA from degradation. Internal ^m6A_m, have also been implicated in regulating gene expression, RNA splicing, and cancer proliferation. We offer the 2’-OMe-N6-Me-A RNA monomer with a phenoxyacetyl protecting group to minimize potential branching. 2’-OMe-N6-Me-A-CE Phosphoramidite is compatible with popular synthesis and deprotection methods, from UltraMild to AMA.

Details

Usage

Coupling: 6 minute coupling time. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: UltraMild 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3105-95	50 µmol	0.47	0.5	3.33	2	1.25	0.91	0.67	0.17
10-3105-90	100 µmol	0.93	1	20	12	7.5	5.45	4	1
10-3105-02	0.25 g	0.25	2.68	0	45.6	28.5	20.73	15.2	3.8

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3105-95	50 µmol	0.05	0.75	0.07	8.6	5.3	3.9	0.5
10-3105-90	100 µmol	0.09	1.5	0.07	23.6	14.7	10.7	1.4
10-3105-02	0.25 g	0.25	4	0.07	73.6	46	33.4	4.6