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1-Me-dA-CE Phosphoramidite - (10-1501)

5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1501

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Product Specifications

Formula:

C₄₂H₄₉ClN₇O₆P

M.W.:

814.31

F.W.:

328.24

CAS Number:

945026-45-9

5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2'-deoxyadenosine) and N3 of cytosine. To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |Monomers that allow for UltraMILD deprotection are recommended. (dA:10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). 0.02M Iodine for oxidation is also recommended.
Deprotection: 2 M Ammonia in methanol, 24 hours at Room Temperature using UltraMILD amidites or 60 hours at Room Temperature if using standard amidites.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	Similar to dA,C,G,T-CE Phosphoramidites

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1501-02	0.25 g	.25grams	3.07	89	53.4	33.38	24.27	17.8	4.45
10-1501-90	100 µmol	.081grams	1	20	12	7.5	5.45	4	1
10-1501-95	50 µmol	.041grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1501-02	0.25 g	.25grams	4.58	0.07	85.2	53.25	38.73	5.33
10-1501-90	100 µmol	.081grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1501-95	50 µmol	.041grams	0.75	0.07	8.6	5.38	3.91	0.54