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DEACM Caged-dG-CE Phosphoramidite - (10-1533)

5'-Dimethoxytrityl-N2-(4-isopropylphenoxyacetyl)-O6-[[7-(diethylamino)coumarin-4-yl]-methyl]-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1533

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Product Specifications

Formula:

C₆₅H₇₅N₈O₁₁P

M.W.:

1175.31

F.W.:

558.49 (DEACM-dG), 329.21 (dG)

CAS Number:

1325717-65-4

5'-Dimethoxytrityl-N2-(4-isopropylphenoxyacetyl)-O6-[[7-(diethylamino)coumarin-4-yl]-methyl]-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Photocaged oligonucleotides allow for the spatial temporal control of biological processes. In their caged form, the oligonucleotides are unable to participate in base pairing. It is only after exposure to non-invasive light that the native oligonucleotide is released. DEACM-dG is a convenient way to introduce a photocage at specific positions, and the DEACM is an effective photocleavable group.

Details

Usage

Coupling: 3 minute coupling time recommended.
Deprotection: If UltraMILD reagents are used, deprotect in 0.05M Potassium Carbonate in Methanol overnight at Room Temperature. If standard bases are used, deprotect in Ammonium Hydroxide at Room Temperature overnight (dmf-dG required) or 30% ammonia/40% methylamine 1:1 (AMA) at Room Temperature for 2 hours (Ac-dC required). Heat should be avoided, both during deprotection and processing.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1533-95	50 µmol	0.06	0.5	3.33	2	1.25	0.91	0.67	0.17
10-1533-90	100 µmol	0.12	1	20	12	7.5	5.45	4	1
10-1533-02	0.25 g	0.25	2.13	57.67	34.6	21.63	15.73	11.53	2.88