Home Products Modification and Labeling Oligonucleotide Modification Reagents Carboxy and NHS-Carboxy Modifier Sequence Modifiers (Carboxy) Carboxy-dT

Carboxy-dT - (10-1035)

5'-Dimethoxytrityl-5-[3-methyl-acrylate]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1035

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Product Specifications

Formula:

C₄₃H₅₁N₄O₁₀P

M.W.:

814.88

F.W.:

360.22

CAS Number:

198080-38-5

5'-Dimethoxytrityl-5-[3-methyl-acrylate]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Cleavage and deprotection should be carried out using a mild 0.4M methanolic sodium hydroxide (methanol:water 4:1) for 17 hours at room temperature. Pipet off support and neutralize with 2M TEAA.|Note: NaOH is not compatible with dmf protecting groups.