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C-TOM-CE Phosphoramidite - (10-3014)

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3014

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Product Specifications

Formula:

C₅₁H₇₂N₅O₁₀PSi

M.W.:

974.22

F.W.:

305.18

CAS Number:

253586-12-8

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.

Details

Usage

Coupling: See technical bulletin for details.
Deprotection: See technical bulletin for details.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3014-02	0.25 g	.25grams	2.57	72.33	43.4	27.13	19.73	14.47	3.62
10-3014-05	0.5 g	.5grams	5.13	157.67	94.6	59.13	43	31.53	7.88
10-3014-10	1.0 g	1grams	10.26	328.67	197.2	123.25	89.64	65.73	16.43

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3014-02	0.25 g	.25grams	3.83	0.07	70.2	43.8	14.63	4.3
10-3014-05	0.5 g	.5grams	7.66	0.07	146.8	71.7	30.58	9.1
10-3014-10	1.0 g	1grams	15.32	0.07	300	187	62.5	18.7