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5'-Biotin II Phosphoramidite - (10-1954)

[1-N-(4,4'-Dimethoxytrityl)-biotinyl-6-aminoethoxyethyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite

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10-1954

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Product Specifications

Formula:

C₄₄H₆₀N₅O₇PS

M.W.:

834.03

F.W.:

393.39

[1-N-(4,4'-Dimethoxytrityl)-biotinyl-6-aminoethoxyethyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite

Description

Biotin is a popular oligonucleotide modification and is used in a wide range of applications, including enrichment, detection, and immobilization assays. 5’-Biotin II consists of an ethylene glycol linker, an alternative structure to our traditional version. 5’-Biotin II phosphoramidite can be added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques.

Details

Usage

Coupling: 2 minute coupling time recommended
Deprotection: 5'-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step. If the final DMT-group is left on to aid in cartridge purification, we recommend that the oligo is left in contact with the TFA solution for 10 minutes.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1954-02	0.25 g	0.25	3	86.67	52	32.5	23.64	17.33	4.33
10-1954-90	100 µmol	0.08	1	20	12	7.5	5.45	4	1
10-1954-95	50 µmol	0.04	0.5	3.3	2	1.25	0.91	0.67	0