Home Products Ancillary Reagents for Oligonucleotide Synthesis Capping Reagents UniCap™ Phosphoramidite UniCap Phosphoramidite

UniCap Phosphoramidite - (10-4410)

Diethyleneglycol ethyl ether (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

As low as $65.00

In stock

Only %1 left

default

10-4410

Product	Options
	To retrieve a catalog number, please select a pack size and format: Copy link to this configuration Size Format See Compatibility Chart 4. Select Your Quantity: Request a quote 3. Price

Product Specifications

Formula:

C₁₅H₃₁N₂O₄P

M.W.:

334.39

CAS Number:

817620-31-8

Diethyleneglycol ethyl ether (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Description

The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.

Details

Usage

Coupling: For capping, add as a second coupling step after base coupling using standard coupling conditions.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	7 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-4410-02	0.25 g	.25grams	7.48	236	141.6	88.5	64.36	47.2	11.8
10-4410-05	0.5 g	.5grams	14.95	485	291	181.88	132.27	97	24.25
10-4410-10	1.0 g	1grams	29.91	983.67	590.2	368.88	268.27	196.73	49.18
10-4410-20	2.0 g	2grams	59.81	1980.33	1188.2	742.63	540.09	396.07	99.02

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-4410-02	0.25 g	.25grams	11.16	0.07	216.8	135.5	98.55	13.55
10-4410-05	0.5 g	.5grams	22.32	0.07	440	275	200	27.5
10-4410-10	1.0 g	1grams	44.63	0.07	886.2	553.88	402.82	55.39
10-4410-20	2.0 g	2grams	89.27	0.07	1779	1111.88	808.64	111.19