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rSpacer TBDMS CE Phosphoramidite
5'-O-Dimethoxytrityl-1'-Deoxyribose-2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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Description
rSpacer is used to introduce an abasic site to an RNA sequence.
Details
Usage
- Coupling: 12 minute coupling recommended
- Deprotection: RNA Deprotection-see bulletin
| Specifications |
| Diluent |
Anhydrous Acetonitrile |
| Storage |
Freezer storage, -10 to -30°C, dry |
| Stability |
1-2 Days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-3915-02 |
0.25 g |
.25grams |
3.33 |
97.67 |
58.6 |
36.63 |
26.64 |
19.53 |
4.88 |
| 10-3915-90 |
100 µmol |
.075grams |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-3915-95 |
50 µmol |
.038grams |
0.5 |
3.33 |
2 |
1.25 |
0.91 |
0.67 |
0.17 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-3915-02 |
0.25 g |
.25grams |
4.97 |
0.07 |
93 |
58.13 |
42.27 |
5.81 |
| 10-3915-90 |
100 µmol |
.075grams |
1.5 |
0.07 |
23.6 |
14.75 |
10.73 |
1.48 |
| 10-3915-95 |
50 µmol |
.038grams |
0.75 |
0.07 |
8.6 |
5.38 |
3.91 |
0.54 |
