The most common side reaction during deprotection of oligonucleotides on a large scale is the alkylation of dT residues by acrylonitrile, formed by ß-elimination of the cyanoethyl phosphate protecting groups, to generate N3-cyanoethyl-dT.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Freezer storage, -10 to -30°C, dry
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.