Ac-dC-Me Phosphonamidite

5'-Dimethoxytrityl-N4-acetyl-2'-deoxyCytidine,3'-[(methyl)-(N,N-diisopropyl)]-phosphonamidite

Product Specifications

Formula:

C₃₉H₄₉N₄O₇P

M.W.:

716.81

F.W.:

287.21

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Technical Documents

Description

Details

Dilution/Coupling Data

Technical Documents

Safety Data Sheet

Synthesis Using Methyl Phosphoramidites

Glen Report 9.12: Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers

Description

Methyl Phosphonamidites may be used in DNA synthesizers following conventional CE Phosphoramidite protocols to produce oligonucleotides containing one or more methyl phosphonate linkages. However, deprotection and purification techniques differ and a description of the procedures is included in the Technical Bulletin. We also offer the dC monomer with acetyl base protection.1 This protecting group is removed with ammonium hydroxide during the cleavage step, eliminating modification at the dC sites during the deprotection step using ethylenediamine in ethanol.

Details

Usage

Coupling: 6 minutes. Note to prevent degradation of the methyl phosphonate linkage, low-water content oxidizer (40-4032) and DMAP for Cap B (40-4020) are recommended.
Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Me-Phosphonamidites.pdf).