UniCap Phosphoramidite

default
10-4410
Diethyleneglycol ethyl ether (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C15H31N2O4P
M.W.:
334.39
Catalog Number: Select Pack Size and Format to Reveal

Description

The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.

Details

Usage

  • Coupling: For capping, add as a second coupling step after base coupling using standard coupling conditions.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 7 days

Intellectual Property

This product is supplied under license.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-4410-02 0.25 g .25grams 7.48 236 141.6 88.5 64.36 47.2 11.8
10-4410-05 0.5 g .5grams 14.95 485 291 181.88 132.27 97 24.25
10-4410-10 1.0 g 1grams 29.91 983.67 590.2 368.88 268.27 196.73 49.18
10-4410-20 2.0 g 2grams 59.81 1980.33 1188.2 742.63 540.09 396.07 99.02

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-4410-02 0.25 g .25grams 11.16 0.07 216.8 135.5 98.55 13.55
10-4410-05 0.5 g .5grams 22.32 0.07 440 275 200 27.5
10-4410-10 1.0 g 1grams 44.63 0.07 886.2 553.88 402.82 55.39
10-4410-20 2.0 g 2grams 89.27 0.07 1779 1111.88 808.64 111.19