U-CE Phosphoramidite

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10-3030
5'-Dimethoxytrityl-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C45H61N4O9PSi
M.W.:
861.06
F.W.:
306.17
CAS Number:
118362-03-1
Catalog Number: Select Pack Size and Format to Reveal

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3030-02 0.25 g .25grams 2.9 83.33 50 31.25 22.73 16.67 4.17
10-3030-05 0.5 g .5grams 5.81 180.33 108.2 67.63 49.18 36.07 9.02
10-3030-10 1.0 g 1grams 11.61 373.67 224.2 140.13 101.91 74.73 18.68

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3030-02 0.25 g .25grams 4.33 0.07 80.2 50.13 16.71 5.01
10-3030-05 0.5 g .5grams 8.67 0.07 167 104.38 34.79 10.44
10-3030-10 1.0 g 1grams 17.33 0.07 340.2 212.63 70.88 21.26