2'-OMe-A-Thiophosphoramidite

5'-Dimethoxytrityl-N-benzoyl-Adenosine, 2'-O-methyl,3'-[(β-thiobenzoylethyl)-(1-pyrrolidinyl)]-thiophosphoramidite
main product photo

Product Specifications

Formula:
C52H53N6O8PS2
M.W.:
985.12
F.W.:
375.36
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet

THIOPHOSPHORAMIDITES AND THEIR USE IN SYNTHESIZING OLIGONUCLEOTIDE PHOSPHORODITHIOATE LINKAGES

Glen Report 27.22: Introducing 2’-OMe-Thiophosphoramidites

Glen Report 34-13: Application Note: Phosphorodithioates in Oligonucleotide Therapeutics

Description

The phosphorodithioate linkage (PS2) is both achiral and essentially resistant to nucleases. Previous studies have shown very interesting results which include observations that DNA with PS2 linkages activates RNase H , strongly inhibits human immunodeficiency virus (HIV) reverse transcriptase, induces B-cell proliferation and differentiation, and is completely resistant to hydrolysis by various nucleases. 2’-OMe- RNA Thiophosphoramidites are RNA monomers designed to produce oligos combining the PS2 linkage with the 2’-O-methyl ribose modification. These PS2-modified RNA oligos have potential for use in siRNAs and dithiophosphate aptamers (thioaptamers).

Details

Usage

  • Coupling: See Technical Bulletin
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Thioamidites2015.pdf).
Specifications
Diluent 10% DCM in Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability Use Immediately

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3170-02 0.25 g .25grams 1.69 43 25.8 16.13 11.73 8.6 2.15
10-3170-90 100 µmol .099grams 0.67 9 5.4 3.38 2.45 1.8 0.45

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3170-02 0.25 g .25grams 2.53 0.1 44.2 27.63 20.09 2.76
10-3170-90 100 µmol .099grams 1 0.1 13.6 8.5 6.18 0.85

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