TAMRA-dT

5'-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C73H83N8O13P
M.W.:
1311.48
F.W.:
870.85
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labelling techniques. Because Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to cleave and deprotect the labelled oligonucleotide must be carefully considered. Using the UltraMILD monomers and deprotection with potassium carbonate in methanol, TAMRA oligonucleotides can be fairly conveniently isolated. To streamline the preparation of TAMRA oligos, we offer 3'-TAMRA CPG for 3' labelling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides.

The spectral characteristics of this dye is detailed here.

Details

Usage

  • Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). Allow 15 minutes for the product to go into solution. A 6 minute coupling time is recommended. Use either dmf-dG (10-1029-xx) with standard monomers and Cap A or use UltraMILD monomers (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ) and UltraMILD Cap Mix A (40-4210-xx/ 40-4212-xx)
  • Deprotection: If standard monomers were used, deprotect in tert-butylamine/water 1:3 (v/v) for 6 hours at 60 °C.  If UltraMILD monomers were used, deprotect in 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_UltraMild_Deprotection.pdf). 
Specifications
Diluent 10% THF in Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1057-02 0.25 g .25grams 1.91 50.33 30.2 18.88 13.73 10.07 2.52
10-1057-90 100 µmol .131grams 1 20 12 7.5 5.45 4 1
10-1057-95 50 µmol .066grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1057-02 0.25 g .25grams 2.85 0.07 50.6 31.63 23 3.16
10-1057-90 100 µmol .131grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1057-95 50 µmol .066grams 0.75 0.07 8.6 5.38 3.91 0.54


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