Sulfurizing Reagent II

default
40-4037

3-((N,N-dimethylaminomethylidene)amino)-3H-1,2,4-dithiazole-5-thione

Product Specifications

Formula:
C5H7N3S3
M.W.:
205.31
CAS Number:
1192027-04-5
Catalog Number: Select Pack Size and Format to Reveal

Description

Glen Research’s Sulfurization Reagents are used to prepare phosphorothioate linkages using CE phosphoramidite chemistry. Each reagent exhibits the following attributes:

  1. Reliably soluble, making them safe to use on automated synthesizers.
  2. Reaction is fast (30 seconds), making the process convenient on small scales and readily amenable to scale-up.
  3. Process is efficient, with better than 96% of the linkages being phosphorothioate and the remainder being phosphodiester. Sulfurizing Reagent (Beaucage Reagent), has been discontnued.

Sulfurizing Reagent II (3-((Dimethylamino-methylidene)amino)-3H-1,2,4-dithiazole-3-thione, DDTT) exhibits all the properties of Beaucage Reagent while adding stability in solution on the synthesizer AND offering strong ability to sulfurize RNA linkages. Sulfurizing Reagent II is available in powder form and as a stable solution.

Details

Usage

  • Coupling: Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
  • Deprotection: See TB_Sulfurizing II.pdf
Specifications
Diluent 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
Storage Controlled room temperature or lower, dry
Stability At least 6 months