Stearyl Phosphoramidite

Octadecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C27H55N2O2P
M.W.:
470.71
F.W.:
332.46
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

We now offer a simple C18 lipid as an economical and effective carrier molecule. We envisage that the 5'-stearyl group will become a favored lipophilic carrier for experimentation with synthetic oligonucleotides.

Details

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1979-02 0.25 g .25grams 5.31 163.67 98.2 61.38 44.64 32.73 8.18
10-1979-90 100 µmol .047grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1979-02 0.25 g .25grams 7.93 0.07 152.2 95.13 69.18 9.51
10-1979-90 100 µmol .047grams 1.5 0.07 23.6 14.75 10.73 1.48