Spermine phosphoramidite is used to produce oligospermine-oligonucleotide conjugates - Zip Nucleic Acids (ZNA®) Oligos. The name reflects the presumed mode of action. The conjugates are believed to use the oligospermine to seek out and move along (scan) oligonucleotide strands until the probe complementary sequence is located. The oligospermine then performs the function of stabilizing the formed duplex by reducing electrostatic repulsion, thereby leading to significantly increased binding affinities. ZNA® Oligos have found use in the following applications: Multiplex PCR; PCR of AT-rich Regions; RT qPCR; Detection of MicroRNA; Improved SNP Discrimination; and Antisense and Antigene Effects. Spermine phosphoramidite is simple to use in oligonucleotide synthesis and can be added multiple times at the 3' or 5' terminus. Deprotection and isolation are also straightforward. HPLC analysis of the conjugates requires high pH to suppress the ionization of the spermine residues.
Coupling: 3 minute coupling time recommended.
Deprotection: Prior to cleavage, treat support with 10% diethylamine in acetonitrile for 5 minutes at room temperature and rinse with acetonitrile to remove the cyanoethyl protecting groups. Then deprotect using 30% ammonium hydroxide as required by nucleobases. (Note a minimum of 4 hours at room temperature, or approximately 30 minutes at 55°C, is required to remove the TFA protecting groups.)
Freezer storage, -10 to -30°C, dry
"Spermine phosphoramidite" synthon is the subject matter ofÂ U.S. Divisional Patent Application 14/745,871, European Patent No. EP 1973927 B1 and foreign equivalents for which Polyplus-transfection is the co-owner. Product is sold for research purposes only. Product shall not be used to manufacture oligospermine oligonucleotide conjugates for use in diagnostics, clinical or commercial applications including use in humans. There is no implied license to manufacture oligospermine oligonucleotide conjugates for diagnostic, clinical or commercial applications, including but not limited to contract research. Please contact Polyplus-transfection atÂ [email protected]Â to obtain a license for such use.