Spermine Phosphoramidite

N¹-[4-(4,4'-Dimethoxytrityloxy)butyl]-N¹,N4,N9,N¹²-tetrakis(trifluoroacetyl)-spermine-N¹²-butyl-4-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C56H73F12N6O9P
M.W.:
1233.17
F.W.:
408.52
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

Spermine phosphoramidite is used to produce oligospermine-oligonucleotide conjugates - Zip Nucleic Acids (ZNA®) Oligos. The name reflects the presumed mode of action. The conjugates are believed to use the oligospermine to seek out and move along (scan) oligonucleotide strands until the probe complementary sequence is located. The oligospermine then performs the function of stabilizing the formed duplex by reducing electrostatic repulsion, thereby leading to significantly increased binding affinities. ZNA® Oligos have found use in the following applications: Multiplex PCR; PCR of AT-rich Regions; RT qPCR; Detection of MicroRNA; Improved SNP Discrimination; and Antisense and Antigene Effects. Spermine phosphoramidite is simple to use in oligonucleotide synthesis and can be added multiple times at the 3' or 5' terminus. Deprotection and isolation are also straightforward. HPLC analysis of the conjugates requires high pH to suppress the ionization of the spermine residues.

Details

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: Prior to cleavage, treat support with 10% diethylamine in acetonitrile for 5 minutes at room temperature and rinse with acetonitrile to remove the cyanoethyl protecting groups. Then deprotect using 30% ammonium hydroxide as required by nucleobases. (Note a minimum of 4 hours at room temperature, or approximately 30 minutes at 55°C, is required to remove the TFA protecting groups.)
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 days

Intellectual Property

"Spermine phosphoramidite" synthon is the subject matter of U.S. Divisional Patent Application 14/745,871, European Patent No. EP 1973927 B1 and foreign equivalents for which Polyplus-transfection is the co-owner. Product is sold for research purposes only. Product shall not be used to manufacture oligospermine oligonucleotide conjugates for use in diagnostics, clinical or commercial applications including use in humans. There is no implied license to manufacture oligospermine oligonucleotide conjugates for diagnostic, clinical or commercial applications, including but not limited to contract research. Please contact Polyplus-transfection at [email protected] to obtain a license for such use.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1939-02 0.25 g .25grams 2.03 54.33 32.6 20.38 14.82 10.87 2.72
10-1939-90 100 µmol .123grams 1 20 12 7.5 5.45 4 1
10-1939-95 50 µmol .062grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1939-02 0.25 g .25grams 3.03 0.07 54.2 33.88 24.64 3.39
10-1939-90 100 µmol .123grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1939-95 50 µmol .062grams 0.75 0.07 8.6 5.38 3.91 0.54