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Spacer Phosphoramidite 18

18-O-Dimethoxytritylhexaethyleneglycol,1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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Product Specifications

Formula:

C₄₂H₆₁N₂O₁₀P

M.W.:

784.93

F.W.:

344.30

CAS Number:

125607-09-2

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Technical Documents

Dilution/Coupling Data

Technical Documents

Safety Data Sheet (North America)

Safety Data Sheet (EU)

Safety Data Sheet (Other)

Glen Report 11.16: More Novel Monomers - 6-Thio-dG, 2'-Amino-RNA, 5-Hydroxy-dC,dU Spacer C18, Spacer 9-CPG

Glen Report 26.11: DNA conjugation to HaloTagged Protein Using Glen Bromohexyl Linker

Glen Report 26.12: Preparation of Bromohexyl-Modified Oligonucleotides for HaloTag Conjugation

Glen Report 7.16: New Modification Reagents and Supports

Description

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3'-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3'-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30°C, dry
Stability	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1918-02	0.25 g	.25grams	3.18	92.67	55.6	34.75	25.27	18.53	4.63
10-1918-90	100 µmol	.078grams	1	20	12	7.5	5.45	4	1

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1918-02	0.25 g	.25grams	4.75	0.07	88.6	55.38	40.27	5.54
10-1918-90	100 µmol	.078grams	1.5	0.07	23.6	14.75	10.73	1.48

References

Novel DNA probes with low background and high hybridization-triggered fluorescence Eugeny A. Lukhtanov, Sergey G. Lokhov, Vladimir V. Gorn, Mikhail A. Podyminogin, and Walt Mahoney

Photosensitized [2 + 2] cycloaddition of N-acetylated cytosine affords stereoselective formation of cyclobutane pyrimidine dimer Junpei Yamamoto, Kosuke Nishiguchi, Koichiro Manabe, Chikahide Masutani, Fumio Hanaoka, and Shigenori Iwai

Ligand Guided Selection (LIGS) with Artificially Expanded Genetic Information Systems against TCR-CD3ε Zumrut, H., Yang, Z., Williams, N., Arizala, J. D. rosario, Batool, S., Benner, S., & Mallikaratchy, P.

Molecular DNA dendron vaccines Max E. Distler, John P. Cavaliere, Michelle H. Teplensky, Michael Evangelopoulos, and Chad A. Mirkin

Light-Controlled Cell_Cell Assembly Using Photocaged Oligonucleotides Katelyn Mathis, Afia Ibnat Kohon, Stephen Black, and Brian Meckes

Transforming Hairpin-like siRNA-Based Spherical Nucleic Acids into Biocompatible Constructs Matthew K. Vasher, Michael Evangelopoulos, and Chad A. Mirkin