Ac-C-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C47H64N5O9PSi
M.W.:
902.11
F.W.:
305.18
CAS Number:
121058-88-6
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet (North America)

Safety Data Sheet (EU)

Safety Data Sheet (Other)

TBDMS-Protected RNA Guide

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 26.14: Technical Brief - AMA – Ubiquitous Reagent for Oligo Deprotection

Glen Report 8.13: New Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers

Glen Report 9.12: Protecting Groups for DNA, RNA and 2'-OMe-RNA Monomers

Glen Report 4-12: RNA Synthesis - Options for 2'-OH Protection

Glen Report 33-14: Application Note – Cleavable Linkages

Glen Report 36-14: Application Note — RNA Synthesis

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

  • Coupling: 12 minute coupling time recommended
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3015-02 0.25 g .25grams 2.77 79 47.4 29.63 21.55 15.8 3.95
10-3015-05 0.5 g .5grams 5.54 171.33 102.8 64.25 46.73 34.27 8.57
10-3015-10 1.0 g 1grams 11.09 356.33 213.8 133.63 97.18 71.27 17.82

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3015-02 0.25 g .25grams 4.14 0.07 76.4 47.7 15.92 4.7
10-3015-05 0.5 g .5grams 8.27 0.07 159 99.3 33.13 9.9
10-3015-10 1.0 g 1grams 16.54 0.07 324 202 67.58 20.2

References

The chemical stability of abasic RNA compared to abasic DNA Pascal A. Kupfer and Christian J. Leumann

DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase Elizabeth Fidalgo da Silva and Linda J. Reha-Krantz

2-Aminopyridine as a Nucleobase Substitute for Adenine in DNA-Like Architectures: Synthesis of Alkynyl C-Nucleotides and their Hybridization Characteristics Kurosaki, F., Chiba, J., Oda, Y., Hino, A., & Inouye, M.

Stalling of Eukaryotic Translesion DNA Polymerases at DNA-Protein Cross-Links Yudkina, A. V., Shilkin, E. S., Makarova, A. V., & Zharkov, D. O.

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