Ribo-tC° Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-1'-(1,3-diaza-2-oxophenoxazin-1-yl)-2’-O-TBDMS-β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C51H64N5O9PSi
M.W.:
950.16
F.W.:
395.26
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

The bright fluorescent tricyclic cytosine analogues tC and tCO stand out among fluorescent bases due to their virtually unquenched fluorescence inside single- or double-stranded DNA. Until recently, this family of tricyclic cytosines had only been studied and used in DNA contexts and, importantly, introduced as possible donors of the first DNA base analogue FRET-pair with tCnitro. Fluorescent base analogues for RNA are limited in number compared to their DNA counterparts. To facilitate the application of such analogues, characterization of their structural and dynamics behavior in RNA compared to the corresponding natural nucleoside is important. We now introduce the tCO ribonucleoside, which has been incorprated into a range of RNA sequences, where it was shown to be a very potent and useful fluorophore in this context.1 Glen Research offers this useful fluorescent ribonucleoside analogue in cooperation with ModyBase HB.

Details

Usage

  • Coupling: 12 minute coupling time recommended.
  • Deprotection: As required by nucleobases. Compatible with ammonium hydroxide/ethanol 3:1 (v/v) for 17 hours at room temperature, AMA (ammonium hydroxide/40% methylamine 1:1 (v/v)) for 10 minutes at 65C or UltraMild deprotection conditions.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30C, dry
Stability 1-2 Days

Intellectual Property

These products are offered in collaboration with ModyBase HB.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3517-02 0.25 g 0.25 2.63 74.33 44.6 27.88 20.27 14.87 3.72
10-3517-90 100 µmol 0.1 1 20 12 7.5 5.45 4 1
10-3517-95 50 µmol 0.05 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3517-02 0.25 g 0.25 3.93 0.07 72.2 45.13 32.82 4.51
10-3517-90 100 µmol 0.1 1.5 0.07 23.6 14.75 10.73 1.48
10-3517-95 50 µmol 0.05 0.75 0.07 8.6 5.38 3.91 0.54

References

(1) Füchtbauer, A.F., Preus, S., Börjesson, K., McPhee, S.A., Lilley D.M.J., Wilhelmsson, L.M., Sci. Rep., 2017, 7, 2393.