The following supports are used to produce oligonucleotides with nuclease resistant 3’-3’ linkages at the 3’ terminus (by attaching regular 3’-CE phosphoramidites) or to produce oligonucleotide sections in the opposite sense (by attaching 5’-CE phosphoramidites). ABI-style columns are supplied unless otherwise requested (see note box).
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in about 5% N4-methyl-dC side product at the modification site.