Ac-dC-5'-CE Phosphoramidite

3'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C41H50N5O8P
M.W.:
771.85
F.W.:
289.18
CAS Number:
1632243-18-5
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet

Glen Report 6.14: 5' To 3' Synthesis

Glen Report 34-14: New Product — Ac-dC-5’-CE Phosphoramidite

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for special applications. The acetyl-protected dC is compatible with all standard deprotection methods.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-5101-02 0.25 g .25grams 3.24 94.67 56.8 35.5 25.82 18.93 4.73
10-5101-05 0.5 g .5grams 6.48 202.67 121.6 76 55.27 40.53 10.13
10-5101-10 1.0 g 1gram 12.96 418.67 251.2 157 114.18 83.73 20.93

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-5101-02E 0.25 g .25grams 4.83 0.07 90.2 56.38 41 5.64
10-5101-05E 0.5 g .5grams 9.67 0.07 187 116.88 85 11.69
10-5101-10E 1.0 g 1gram 19.34 0.07 380.4 237.75 172.91 23.78

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