Glen Report 36-13: New Product —5X Tris-Borate-EDTA (TBE) Buffer

We offer a few buffers already that are used for oligonucleotide processing, analysis, and purification. A couple of these are ammonium acetate buffers, triethylammonium acetate (TEAA) and hexylammonium acetate (HAA), commonly used as mobile phase buffers for reverse phase high-performance liquid chromatography (RP-HPLC). We also have a quenching buffer that is used to stop the 2′-desilylation reaction for RNAs. We are happy to expand our buffer offerings by introducing 5X Tris-Borate-EDTA (TBE) Buffer.

When diluted to 1X working concentration, TBE is a popular running buffer for various applications:

  • Electrophoresis of nucleic acids in agarose and polyacrylamide gels analyzing RNA and DNA fragments smaller than 1,500 bp1
  • Plasmid characterization and extraction
  • DNA automated sequencing gels
  • DNA fingerprinting
  • Detection of DNA polymorphisms
  • Restriction enzyme mapping2

This electrophoresis buffer is essential for the success of agarose gels and polyacrylamide gel electrophoresis (PAGE) because it allows current to flow through the gel while keeping oligonucleotide samples at biologically appropriate pH to maintain their charge and structure.3
It is compatible with both non-denaturing and denaturing gels and yields higher resolution of smaller fragments. TBE has a higher buffer capacity, compared to other running buffers, meaning it performs better over a longer period of time.

Our TBE is made with high quality reagents and is tested to ensure the buffer is free of RNase contamination.
Once diluted 5-fold, TBE buffer contains 89 mM Tris Borate and 2 mM EDTA at pH 8.3 and is ready for use in gel electrophoresis.

For successful gel electrophoresis experiments when using our 1X TBE buffer, we generally recommend following some tips:

  • Load equal volumes of DNA and ladder DNA
  • Stick to using one loading dye per gel
  • Avoid high salt concentrations in your samples



  1. R.C. Ogden, and D.A. Adams, Methods Enzymol, 1987, 152, 61-87.
  2. E. Southern, Methods Enzymol, 1979, 68, 152-76.
  3. B.A. Sanderson, N. Araki, J.L. Lilley, G. Guerrero, and L.K. Lewis, Anal Biochem, 2014, 454, 44-52.

Product Information

5X Tris-Borate-EDTA (TBE) Buffer (60-4130)