We are happy to offer several products containing the BlackBerry. Quencher (BBQ-650.), which exhibits a broad absorption profile from 550nm to 750nm, centered at 650nm. This range offers more effective quenching of some of our popular long wavelength dyes like TAMRA, Redmond Red, Cy dyes and DyLight dyes. We offer BBQ-650 products for the 3' and 5' termini, as well as BBQ-650-dT for inclusion within the oligonucleotide sequence, with the following properties:
Quenches the fluorescence of long wavelength dyes
Quenches in FRET and contact mode
Absorbance maximum at ~650nm
Quenching range - 550-750nm
Compatible with standard oligo synthesis chemistry
Compatible with regular deprotection but requires mild deprotection with AMA at room temperature
Available for 3', 5', and internal substitution
More stable than BHQ-3
Details
Usage
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Complete the deprotection using the protocol required by the nucleobases. Compatible with standard deprotection conditions in Ammonium Hydroxide and room temperature AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) for 2 hours.
Specifications
Diluent
Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Storage
Freezer storage, -10 to -30°C, dry. Light sensitive material.
Stability
1-2 days
Intellectual Property
BlackBerry® Quencher technology: US Patent 7,879,986. The purchase of BlackBerry® Quencher reagents includes a limited license to use these reagents exclusively for research and development purposes. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed, or re-packaged without prior agreement and consent of Berry & Associates, Inc. Subsequent sale of products that are derived from BlackBerry® Quencher reagents is permitted so long as the following written disclaimer is included in written and electronic catalogs, in commercial advertisement, and in packages with containers of such derivative products: "BlackBerry is a trademark of Berry & Associates, Inc. Products derived from BlackBerry® Quencher reagents are sold exclusively for research and development use by the purchaser. They may not be used for clinical or diagnostic purposes without prior agreement and consent of Berry & Associates, Inc."
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
