Pyrrolo-dC-CE Phosphoramidite

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10-1017
5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2'-deoxyribonucleoside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C42H50N5O7P
M.W.:
767.85
F.W.:
327.23
CAS Number:
644962-92-5
Catalog Number: Select Pack Size and Format to Reveal

Description

Pyrrolo-dC is a fluorescent deoxycytidine analog that is an ideal probe of DNA structure and dynamics. It base-pairs as a normal dC nucleotide. An oligo fully substituted with pyrrolo-dC has the same Tm as the control dC oligo with the same specificity for dG. Its small size does not perturb the structure of the DNA helix and it is well tolerated by a number of DNA and RNA polymerases. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-dCTP has potential uses in biological assay development.

Details

Usage

  • Coupling: Standard Coupling time. Use 0.02 M Iodine for Oxidation. Note: Use 0.5 M CSO in anhydrous ACN (40-4632-xx) and a 3 minute oxidation time for best results with multiple additions.
  • Deprotection: Ammonium Hydroxide for 24 hours at room temperature.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 2-3 days

Intellectual Property

This product is covered by US Patent No.: 7,144,995.|Patented under European patent number 1483280.


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1017-02 0.25 g .25grams 3.26 95.33 57.2 35.75 26 19.07 4.77
10-1017-90 100 µmol .077grams 1 20 12 7.5 5.45 4 1
10-1017-95 50 µmol .038grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1017-02 0.25 g .25grams 4.86 0.07 90.8 56.75 41.27 5.68
10-1017-90 100 µmol .077grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1017-95 50 µmol .038grams 0.75 0.07 8.6 5.38 3.91 0.54