Psoralen C2 Phosphoramidite

default
10-1982
2-[4'-(hydroxymethyl)-4,5',8-trimethylpsoralen]-ethyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula:
C26H35N2O6P
M.W.:
502.55
F.W.:
364.29
CAS Number:
126221-83-8
Catalog: Make Selections to Reveal

Description

Psoralen C2 at the 5’-terminus of an oligonucleotide serves effectively as a cross-linking reagent in double-stranded oligonucleotides. The 6 atom spacer arm of Psoralen C6 allows cross-linking with a triplex oligonucleotide strand. Click Chemistry with psoralen azide and one of our many nucleosidic and non-nucleosidic alkyne derivatives has the potential to generate a variety of practical cross-linkers. The well known reversible cross-linking behavior of psoralen with an adjacent thymidine residue could be very useful.

Details

Usage

  • Coupling: 10 minute coupling time recommended.
  • Deprotection: Mild: 0.4M Methanolic NaOH 17hr @ RT or ammonium hydroxide 24h @ RT.|Note: NaOH is not compatible with dmf protecting groups.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1982-02 0.25 g .25grams 4.97 152.33 91.4 57.13 41.55 30.47 7.62
10-1982-90 100 µmol .05grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1982-02 0.25 g .25grams 7.42 0.07 142 88.75 64.55 8.88
10-1982-90 100 µmol .05grams 1.5 0.07 23.6 14.75 10.73 1.48


Product FAQs

  • Which should I use - psoralen C2 or psoralen C6?
  • What is the sequence requirement for psoralen mediated cross-linking in double stranded DNA?