PseudoUridine-CE Phosphoramidite

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10-3055
5'-Dimethoxytrityl-pseudoUridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

Formula:
C45H61N4O9PSi
M.W.:
861.05
F.W.:
306.17
CAS Number:
163496-23-9
Catalog Number: Select Pack Size and Format to Reveal

Description

PseudoUridine is one of the most common modified nucleosides found in RNA. The availability of a phosphoramidite will allow detailed research into the effects of this modified base on RNA structure and activity.

Details

Usage

  • Coupling: 12 minute coupling time recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days


Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3055-02 0.25 g .25grams 2.9 83.33 50 31.25 22.73 16.67 4.17
10-3055-90 100 µmol .086grams 1 20 12 7.5 5.45 4 1
10-3055-95 50 µmol .043grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3055-02 0.25 g .25grams 4.33 0.07 0 0 16.71 0
10-3055-90 100 µmol .086grams 1.5 0.07 0 0 4.92 0
10-3055-95 50 µmol .043grams 0.75 0.07 0 0 1.79 0