Dabcyl Labeling

 A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence.  Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer.  The Dabcyl group has been found to be a universal quencher.  3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus.  5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.

2 Product Results
 
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  1. Sugar Type Deoxyribose Remove This Item
  • Dabcyl-dT
    Dabcyl-dT (10-1058)
    Catalog #: 10-1058  |  CAS #: 316121-62-7
    Formal Name: 5'-Dimethoxytrityloxy-5-[(N-4'-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • 5'-Dabsyl-dT-CE Phosphoramidite
    5'-Dabsyl-dT-CE Phosphoramidite (10-1532)
    Catalog #: 10-1532
    Formal Name: 5'-Dabsyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
 
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