Dabcyl Labeling

 A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence.  Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer.  The Dabcyl group has been found to be a universal quencher.  3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus.  5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.

4 Product Results
 
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  1. Label/Modifier Type Dabcyl Remove This Item
  • Dabcyl-dT
    Dabcyl-dT (10-1058)
    Catalog #: 10-1058  |  CAS #: 316121-62-7
    Formal Name: 5'-Dimethoxytrityloxy-5-[(N-4'-carboxy-4-(dimethylamino)-azobenzene)-aminohexyl-3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • 5'-Dabcyl Phosphoramidite
    5'-Dabcyl Phosphoramidite (10-5912)
    Catalog #: 10-5912  |  CAS #: 288631-58-3
    Formal Name: 6-(N-4'-carboxy-4-(dimethylamino)azobenzene)-aminohexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
  • 3'-Dabcyl CPG
    3'-Dabcyl CPG (20-5912)
    Catalog #: 20-5912
    Formal Name: 1-Dimethoxytrityloxy-3-[O-(N-4'-carboxy-4-(dimethylamino)-azobenzene)-3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG
  • 3'-Dabcyl PS
    3'-Dabcyl PS (26-5912)
    Catalog #: 26-5912
    Formal Name: 1-Dimethoxytrityloxy-3-[O-(N-4'-carboxy-4-(dimethylamino)-azobenzene)-3-aminopropyl)]-propyl-2-O-succinoyl-polystyrene
 
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