Minor RNA Triphosphates

Pyrrolo-dC is a fluorescent nucleoside that codes as dC and base pairs efficiently with dG.  Preliminary evidence indicates that pyrrolo-dC triphosphate is an excellent substrate for Taq, Pfu and Vent polymerases and is incorporated specifically opposite dG. Pyrrolo-dCTP has been available for some time and is in use in biological assays.  Pyrrolo-CTP is a fluorescent ribonucleotide with fluorescence exquisitely sensitive to its environment and is of great interest for RNA structural research.  The pyrrolo-C project is a joint development by Berry and Associates, Inc. and Glen Research Corporation. 

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