Analog Phosphoramidites for Photo-Regulation of DNA Function

Glen Research’s interest lies in the preparation of caged oligonucleotides whose function is restored after uncaging by UV light at a wavelength that causes no DNA damage.  The Deiters group at North Carolina State University has described NPOM-Caged-dT, where the nucleobase is caged with the photolabile group, 6-nitropiperonyloxymethyl (NPOM), which can be removed using UV light at 365nm.  Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand.  Photo-uncaging of the caged oligonucleotide is then easily carried out with UV light at 365 nm for seconds to minutes to restore the activity of the oligonucleotide.

1 Product Results
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  • NPOM Caged-dT-CE Phosphoramidite
    NPOM Caged-dT-CE Phosphoramidite (10-1534)
    Catalog #: 10-1534  |  CAS #: 942218-71-5
    Formal Name: 5'-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2'-deoxyThymidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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