Analog Phosphoramidites for Mutagenesis in Oligonucleotide Synthesis

Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone.  In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2’-deoxyadenosine) and N3 of cytosine.  To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.

5 Product Results
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  • O6-Me-dG-CE Phosphoramidite
    O6-Me-dG-CE Phosphoramidite (10-1070)
    Catalog #: 10-1070  |  CAS #: 117958-69-7
    Formal Name: 5'-Dimethoxytrityl-N-isobutyryl-O6-methyl-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • N6-Me-dA-CE Phosphoramidite
    N6-Me-dA-CE Phosphoramidite (10-1003)
    Catalog #: 10-1003  |  CAS #: 105931-58-6
    Formal Name: 5'-Dimethoxytrityl-N6-methyl-2'-deoxyAdenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • N6-Ac-N6-Me-dA-CE Phosphoramidite
    N6-Ac-N6-Me-dA-CE Phosphoramidite (10-1503)
    Catalog #: 10-1503
    Formal Name: 5'-Dimethoxytrityl-N6-acetyl-N6-methyl-2'-deoxyAdenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • O4-Me-dT-CE Phosphoramidite
    O4-Me-dT-CE Phosphoramidite (10-1032)
    Catalog #: 10-1032  |  CAS #: 130583-06-1
    Formal Name: 5'-Dimethoxytrityl-O4-methyl-2'-deoxyThymidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • 1-Me-dA-CE Phosphoramidite
    1-Me-dA-CE Phosphoramidite (10-1501)
    Catalog #: 10-1501  |  CAS #:  945026-45-9
    Formal Name: 5'-Monomethoxytrityl-N6-chloroacetyl-1-methyl-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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