CleanAmp™ Monomers

CleanAmp™ Primers offer an alternative to other Hot Start technologies and allow greater control of primer hybridization and extension during PCR. It has been demonstrated that CleanAmp Primers outperform other technologies in multiple applications. Indeed, over a broad range of applications, CleanAmp Primers reduce or eliminate off-target amplification. Greater amplicon yield is also achieved, due to improvement in specificity and sensitivity.  By using either the slow-releasing Precision primers with two CleanAmp phosphotriester linkages or the faster-releasing Turbo Primers with a single CleanAmp phosphotriester linkage, the rate of formation of unmodified primer can be controlled to suit reaction needs.  A table to aid in the selection of Turbo and Precision Primers for specific applications is shown below.

Turbo Primers

Precision Primers

Fast cycling

Standard cycling

Multiplex PCR

One-step reverse-transcription PCR

Improves amplicon yield

Improved specificity and limit of detection

Reduces mis-priming/ primer dimer formation

Greatest reduction in mis-priming/primer dimer formation

Synthesis of CleanAmp Primers requires the use of UltraMild Chemistry.

CleanAmp™ Primers and monomers are available from TriLink BioTechnologies.

INTELLECTUAL PROPERTY

CleanAmp™ is a trademark of TriLink BioTechnologies, Inc. CleanAmp™ products or portions thereof are covered by TriLink pending Patent Applications, US 2007281308 and WO2007139723, US Provisional Patent Application Serial # 61/056,324 and US Patent 6762298 licensed from the Department of Health and Human Services. CleanAmp™ products are sold exclusively for R & D use by the purchaser. They may not be resold, distributed or re-packaged. No license is granted or implied with the purchase of this product. Amplification methods used in connection with Polymerase Chain Reaction (“PCR”) Process are covered by many patents. It may be necessary to obtain a separate license for certain patented applications in which the product is used. CleanAmp™ Licenses are available directly from TriLink BioTechnologies. www.trilinkbiotech.com

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