UltraMild TBDMS-Protected RNA Phosphoramidites

An alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels.  A set of monomers using phenoxyacetyl (Pac) protected A and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected G, along with acetyl protected C, met the desired criteria for UltraMILD deprotection.

We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A.  This modification removes the possibility of exchange of the iPr-Pac protecting group on the G with acetate from the acetic anhydride capping mix.  Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.

4 Product Results
 
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  • Pac-A-CE Phosphoramidite
    Pac-A-CE Phosphoramidite (10-3000)
    Catalog #: 10-3000
    Formal Name: 5'-Dimethoxytrityl-N-phenoxyacetyl-Adenosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • Ac-C-CE Phosphoramidite
    Ac-C-CE Phosphoramidite (10-3015)
    Catalog #: 10-3015  |  CAS #: 121058-88-6
    Formal Name: 5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • iPr-Pac-G-CE Phosphoramidite
    iPr-Pac-G-CE Phosphoramidite (10-3021)
    Catalog #: 10-3021
    Formal Name: 5'-Dimethoxytrityl-N-p-isopropylphenoxyacetyl-Guanosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • U-CE Phosphoramidite
    U-CE Phosphoramidite (10-3030)
    Catalog #: 10-3030  |  CAS #: 118362-03-1
    Formal Name: 5'-Dimethoxytrityl-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
 
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