The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3'-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3'-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers.
Coupling: 2 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Freezer storage, -10 to -30°C, dry
Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., +617-975-0680, http://www.ambergen.com/.
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.